shortcut rnaseiii (New England Biolabs)
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Shortcut Rnaseiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnaseiii/ShortCut+RNase+III/pm41571440-161-3-5
Average 96 stars, based on 316 article reviews
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other:Article Title: Functional analysis of a random-sequence chromosome reveals a high level and the molecular nature of transcriptional noise in yeast cells. Article Snippet: Article In Vitro:Article Title: Purification/Amplification-Free RNA Detection Platform for Rapid and Multiplex Diagnosis of Plant Viral Infections. Article Snippet: Genetic tests are highly sensitive, and quantitative methods for diagnosing human viral infections, including COVID-19, are also being used to diagnose plant diseases in various agricultural settings.. Conventional genetic tests for plant viruses are mostly based on methods that require purification and amplification of viral genomes from plant samples, which generally take several hours in total, making it difficult to use them in rapid detection at point-of-care testing (POCT).. In this study, we developed Direct-SATORI, a rapid and robust genetic test that eliminates the purification and amplification processes of viral genomes by extending the recently developed amplification-free digital RNA detection platform called SATORI, allowing the detection of various plant viral genes in a total of less than 15 min with a limit of detection (LoD) of 98 ∼ copies/μL using tomato viruses as an example. Incubation:Article Title: Purification/Amplification-Free RNA Detection Platform for Rapid and Multiplex Diagnosis of Plant Viral Infections. Article Snippet: Genetic tests are highly sensitive, and quantitative methods for diagnosing human viral infections, including COVID-19, are also being used to diagnose plant diseases in various agricultural settings.. Conventional genetic tests for plant viruses are mostly based on methods that require purification and amplification of viral genomes from plant samples, which generally take several hours in total, making it difficult to use them in rapid detection at point-of-care testing (POCT).. In this study, we developed Direct-SATORI, a rapid and robust genetic test that eliminates the purification and amplification processes of viral genomes by extending the recently developed amplification-free digital RNA detection platform called SATORI, allowing the detection of various plant viral genes in a total of less than 15 min with a limit of detection (LoD) of 98 ∼ copies/μL using tomato viruses as an example. Article Title: Exploring fluoropolymers for fabrication of femtoliter chamber arrays used in digital bioanalysis Article Snippet: .. To remove the double-stranded RNA contaminations, the transcribed RNA was incubated with Purification:Article Title: Purification/Amplification-Free RNA Detection Platform for Rapid and Multiplex Diagnosis of Plant Viral Infections. Article Snippet: Genetic tests are highly sensitive, and quantitative methods for diagnosing human viral infections, including COVID-19, are also being used to diagnose plant diseases in various agricultural settings.. Conventional genetic tests for plant viruses are mostly based on methods that require purification and amplification of viral genomes from plant samples, which generally take several hours in total, making it difficult to use them in rapid detection at point-of-care testing (POCT).. In this study, we developed Direct-SATORI, a rapid and robust genetic test that eliminates the purification and amplification processes of viral genomes by extending the recently developed amplification-free digital RNA detection platform called SATORI, allowing the detection of various plant viral genes in a total of less than 15 min with a limit of detection (LoD) of 98 ∼ copies/μL using tomato viruses as an example. Article Title: Transcription elongation defects link oncogenic splicing factor mutations to targetable alterations in chromatin landscape Article Snippet: .. The eluted chromatin was digested for one hour in Article Title: Exploring fluoropolymers for fabrication of femtoliter chamber arrays used in digital bioanalysis Article Snippet: .. To remove the double-stranded RNA contaminations, the transcribed RNA was incubated with Article Title: Synergistic combination of RAD51-SCR7 improves CRISPR-Cas9 genome editing efficiency by preventing R-loop accumulation Article Snippet: .. 10 μg of this genomic DNA from HEK293T cells was digested with 50 U EcoRI-HF (NEB, R3101S) and 50 U PvuII-HF (NEB, R3151S) in NEB CutSmart buffer at 37°C for 4 h. To remove RNA moieties, the products were then digested with 0.25 μg/mL RNase A (Thermo Scientific, EN0531), 25 U/ml RNase T1 (Thermo Scientific, EN0541), 25 U/ml Article Title: Synergistic combination of RAD51-SCR7 improves CRISPR-Cas9 genome editing efficiency by preventing R-loop accumulation. Article Snippet: .. 10 mg of this genomic DNA from HEK293T cells was digested with 50 U EcoRI-HF (NEB, R3101S) and 50 U PvuII-HF (NEB, R3151S) in NEB CutSmart buffer at 37 C for 4 h. To remove RNA moieties, the products were then digested with 0.25 mg/mL RNase A (Thermo Scientific, EN0531), 25 U/ml RNase T1 (Thermo Scientific, EN0541), 25 U/ml Polyacrylamide Gel Electrophoresis:Article Title: Purification/Amplification-Free RNA Detection Platform for Rapid and Multiplex Diagnosis of Plant Viral Infections. Article Snippet: Genetic tests are highly sensitive, and quantitative methods for diagnosing human viral infections, including COVID-19, are also being used to diagnose plant diseases in various agricultural settings.. Conventional genetic tests for plant viruses are mostly based on methods that require purification and amplification of viral genomes from plant samples, which generally take several hours in total, making it difficult to use them in rapid detection at point-of-care testing (POCT).. In this study, we developed Direct-SATORI, a rapid and robust genetic test that eliminates the purification and amplification processes of viral genomes by extending the recently developed amplification-free digital RNA detection platform called SATORI, allowing the detection of various plant viral genes in a total of less than 15 min with a limit of detection (LoD) of 98 ∼ copies/μL using tomato viruses as an example. Article Title: Exploring fluoropolymers for fabrication of femtoliter chamber arrays used in digital bioanalysis Article Snippet: .. To remove the double-stranded RNA contaminations, the transcribed RNA was incubated with Sterility:Article Title: Chronic viral mimicry induction following p53 loss promotes immune evasion Article Snippet: .. Double digestion reactions were prepared as follows: 1 μg cytosolic nucleic acids, 1x NEB ShortCutBuffer, 20mM MnCl 2 , Polymerase Chain Reaction:Article Title: Transcription elongation defects link oncogenic splicing factor mutations to targetable alterations in chromatin landscape Article Snippet: .. The eluted chromatin was digested for one hour in Gel Extraction:Article Title: Synergistic combination of RAD51-SCR7 improves CRISPR-Cas9 genome editing efficiency by preventing R-loop accumulation Article Snippet: .. 10 μg of this genomic DNA from HEK293T cells was digested with 50 U EcoRI-HF (NEB, R3101S) and 50 U PvuII-HF (NEB, R3151S) in NEB CutSmart buffer at 37°C for 4 h. To remove RNA moieties, the products were then digested with 0.25 μg/mL RNase A (Thermo Scientific, EN0531), 25 U/ml RNase T1 (Thermo Scientific, EN0541), 25 U/ml Article Title: Synergistic combination of RAD51-SCR7 improves CRISPR-Cas9 genome editing efficiency by preventing R-loop accumulation. Article Snippet: .. 10 mg of this genomic DNA from HEK293T cells was digested with 50 U EcoRI-HF (NEB, R3101S) and 50 U PvuII-HF (NEB, R3151S) in NEB CutSmart buffer at 37 C for 4 h. To remove RNA moieties, the products were then digested with 0.25 mg/mL RNase A (Thermo Scientific, EN0531), 25 U/ml RNase T1 (Thermo Scientific, EN0541), 25 U/ml |
